The 29th Battalion was originally formed during the First World War, being raised in Victoria as part of the Australian Imperial Force (AIF) on 10 August 1915.
19 October 1918, the 29th Battalion was disbanded.
The battalion was re-raised in 1921, becoming a part-time unit of the Citizen Forces, assigned to the 4th Brigade, 3rd Division.
In 1927, territorial titles were introduced into the Australian Army and the battalion adopted the title of the "East Melbourne Regiment". At this time, the battalion was afforded the motto Nulli Secundus.
In 1930 the 29th Battalion was amalgamated with the 22nd, forming the 29th/22nd Battalion, although they were later split again in August 1939 and the 29th re-raised in its own right.
In August 1942, the 29th Battalion was amalgamated with the 46th Battalion to form the 29th/46th Battalion. This unit went on to serve overseas in New Guinea and on New Britain.
After the war, following the demobilisation of the wartime Army, Australia's part-time military was re-formed in 1948, but the 29th Battalion was not re-raised.